BRAF antibody (AA 25-75)

Catalog No. ABIN1977055

This Rabbit Polyclonal antibody specifically detects BRAF in WB, IHC, IF, IHC (p) and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog and Cow.

Quick Overview for BRAF antibody (AA 25-75) (ABIN1977055)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-BRAF Antibody

Binding Specificity: AA 25-75

Specificity: Region between residue 25 and 75 of human v-raf murine sarcoma viral oncogene homolog B1 using the numbering given in entry NP_004324.2 (GeneID 673).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:1000), IHC, IHC-P (1:200 - 1:1000), IP (2 - 5 μg/mg lysate), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Prostate Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF / B-Raf

Background: Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1

Gene ID: 673

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy